Oxytocin concentration in Liquid Trust??

[Archetypical Hybrid (HEC)]

Does anyone know definitively of the concentration (either IU or mg/ml) of oxytocin in liquid

trust??

I wonder if they use synthetic (>95% purity) or enzymatically-producted oxytocin..

Can someone shed

some light on the subject please?

[WorkingMann]

I would say it cant be much

if you look at the following

link:
http://www.prospec.co.il/~prospec/cart/customer/search.php?substring=oxyt&source=GoogleA

d&agent_camp=8238001&agent_bann=8241001 (http://www.prospec.co.il/%7Eprospec/cart/customer/search.php?substring=oxyt&source=GoogleAd&agent_camp=

8238001&agent_bann=8241001)

Our price: $ 50.00 /5 mg of Human Oxytin and with

the price were giving it can't be high.. But as many other "pheroes" (I dont know if I can call oxytin a phero)

small amounts is needed else it will cause terrible results like OD'in..

And if you read further down:


Lyophilized Oxytocin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C.

Upon reconstitution Oxytocin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term

storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Does this mean that our LT only

lasts 3 weeks in room temperature? And if we wanna keep it more than 3 weeks it should be used after that it should

be stored between 4 and -18 degree Celcius?

[Archetypical Hybrid (HEC)]

Wow.. thanks

for that link! we had done some with with oxytocin and got it from Sigma-Aldrich for $138 / 5mg..

RE: storage

temp: this is the storage temp if it's stored as a powder. If it's dissolved in a solution (I.E. liquid trust)

then this is no longer applicable.

I still need to know of the concentration of oxytocin in LT.. anyone??

[gaf]

Read this interview with one of the

dudes from vero labs , he states the concentration is a "trade secret"



http://kuchinskas.typepad.com/hug_the_monkey/2006/08/interview_with_.html

[xvs]

My guess is that the concentration is

"homeopathic".

In other words, almost no oxytocin at all.

[WorkingMann]

Wow.. thanks for that link! we had done some with with oxytocin and got it from Sigma-Aldrich for $138 / 5mg..



RE: storage temp: this is the storage temp if it's stored as a powder. If it's dissolved in a solution (I.E.

liquid trust) then this is no longer applicable.

I still need to know of the concentration of oxytocin in LT..

anyone??

But will the oxytin still "live/survive" room temperature after a month in this "heat"?

[slickracer]

Wow.. thanks for that link! we had done some with with oxytocin and got it from Sigma-Aldrich for $138 / 5mg..



RE: storage temp: this is the storage temp if it's stored as a powder. If it's dissolved in a solution (I.E.

liquid trust) then this is no longer applicable.

I still need to know of the concentration of oxytocin in LT..

anyone??

wow thats really intresting! so do you think LT will last pretty long, if i just left it in my

car for a while?

oh and , what else are you brewing up right now? btw

[Archetypical Hybrid (HEC)]

oh and

, what else are you brewing up right now? btw

As many people report major success with certain

combinations, the current school of thought is to fuse such combinations together into one convenient package..(I.e.

AQ + LT)

BTW slick... AQ is totally different from Chikara - different formula, different manufacturer,

etc..

**Does anyone know how to quantify the concentration of a peptide-complex?? *scratches head*

[slickracer]

if i may sugest something, do

you think you can you can brew something up thats sorta like a combanation of AE + masters.

its just a

suggestion and it dosn't really matter if you make it or not as i do my own mixing with it. but i just like to say

that, its a really good mix. i think thats just what im going to buy from now on, as i havn't found anything else

thats better then this (i'll also buy NPA of course) and the last couple of things i brough hasn't really worked

for me.

here is the break down of the stuff i have tried.

ae, masters, NPA= love them

chikara = its

alright makes me feel like a smart ass and i like to teach people stuff when i wear it

KZI testing kit = fave

one was the 5:1 as i got some decent results with it so i migh try that alpha 7 down the road.

lucky 7 chem set

= awsome as i can make anything with it, i ended up makeing my own ae (2:1.5:1) with a dash of other mones like A1

and estra. but i had to order the real ae and other stuff as my lid for the nol broke because it was on too tight

and now i got no more nol.

wagg and soe= did what it was suppose to do, but their vibes and my personality

didn't match that much.

LT + TE = still messing around with it, i see some potential but still havn't found

the right dose.

[Archetypical Hybrid (HEC)]

NOTE THAT

i'VE STARTED A NEW THREAD FOR NEW PRODUCT SUGGESTIONS.. Please post all suggestions in this thread!

[Archetypical Hybrid (HEC)]

Slick: do you

think that you or others would be interested in some free reagent-grade-solution mones, to facilitate any

development of new formulas to which you could report? (I.e. 25ml of nol Vs. 5ml chem-set?) - this would probably

require a NDA...

EDIT: So, in a nutshell those who qualify could be sent a free "development-kit,"

directly from love-scent with Bruce's approval..

This would include:

- NON-DISCLOSURE AGREEMENT

-

25ml of each of the mones
- 50ml of SDA40B ethanol
- 100ml of propylene glycol
- Test tubes / Vials
- Mixing

bottles
- Pipettes / extraction bulbs
- Designer cologne oils
- Small notepad to record results

P.S. This

isn't stamped and approved yet.. Awaiting Bruce's authorization :thumbsup:

P.S.S. It might even be structured

so that a small deposit might be paid to love-scent initially, which could be refunded upon return-receipt of the

notepad..

[slickracer]

sure, i would love to help,

and i have alot of experiance working with mones now.

if you want to send me the contract after i give you my

email (which will be around friday as i got to go througe my old acount still) i'll look over that stuff and see

where it goes from there.


edit* oh yeah, also, if you can sum up how to use the ethonal and mones in like a

little paragraph it would be great (use examples if you can cause i can comprehand things better with examples) as

to tell you the truth, i dont have any experiance doing that.

[WorkingMann]

hey HEC you didn't

answer
But will the oxytin still "live/survive" room temperature after a month in this "heat"?
:blink:
This is

because I don't want to ny LT goes to waste.. Im here talking about my LT where its liquid..

[Archetypical Hybrid (HEC)]

To be totally

honest I believe the effect of liquid trust is due to the placebo effect.. Oxytocin has really only been shown to be

active when it is directly inhaled nasally as a spray (and is a prescription only drug when used as such).. It's a

very large, conglomerate molecule and so it would be very difficult to get it into the air as a vapor..

Does it

actually work as marketed? I find it hard to believe.

But as far as stability: Yes, high temps should be

avoided, as the protiens in peptide-complexes (I.e. oxytocin) often denature at higher temperatures..

I should think that Vero Labs would have incorporated some sort of preservative to hinder this, but it seems not...

So in a nutshell avoid high temperatures and direct sunlight exposure.

de·na·ture

(dē-nā'chər)

http://content.answers.com/main/content/img/pron.gif
tr.v., -tured, -tur·ing,

-tures.
Biochemistry.
To cause the tertiary structure of (a protein) to unfold, as

with heat, alkali, or acid, so that some of its original properties, especially its biological activity, are

diminished or eliminated.

To preserve maximum stability, I would recommend storage in a household

freezer (the formula contains alcohol, so it won't freeze to ice). Don't leave it in your car's glovebox for very

long and expect it to still be active (if it even is at all from the get-go).

[xvs]

But as far as stability: Yes, high temps should be avoided, as the protiens in peptide-complexes (I.e.

oxytocin) often denature at higher temperatures..

...

To cause the tertiary structure

of (a protein) to unfold...

Oxytocin is not a protein. It is a peptide made of only 9 amino acids.



It has no tertiary structure.

[WorkingMann]

Oxytocin is not a

protein. It is a peptide made of only 9 amino acids.

It has no tertiary structure.

I think HEC meant

the proteins in the peptide..



But as far as stability: Yes, high temps should

be avoided, as the protiens in peptide-complexes (I.e. oxytocin) often denature at

higher temperatures..

[Mungojerry]

ddddddddddddddddddddddddddddddd

[jvkohl]

There are no

proteins in peptides.

Essentially, peptides are short molecules formed by linkage of amino acids. Protiens

are long molecules formed by linkage of amino acids.

Hence you don't have proteins in

peptides.

Gentlemen,
Having been acused on several occassions of getting "too technical," I

suspect we may be pressing the upper limits of technicalities here, but also wanted to add:

From Wikpedia:

(because even I had to check my assumptions)
"a peptide is an amino acid molecule without secondary structure; on

gaining defined structure, it is a protein." Thus the same molecule can be either a peptide or a protein depending

on its environment, though there are peptides that cannot be

proteins."

JVK

[Whitehall]

Either get digested in the human

gut. That allows us to enjoy a big steak but means we can't take oxytocin orally.

[jvkohl]

Either get

digested in the human gut. That allows us to enjoy a big steak but means we can't take oxytocin orally.



Thus, the intranasal route, which takes us back to the question of concentration of oxytocin in the product. If

the product was developed in accord with human studies, shouldn't the concentration be obvious--whatever

concentration was used in the human studies, for example?



JVK
(http://)

[bronzie]

this product has done jack for

me, as hec mentioned, i believe the only benefit is a placebo effect

great atomizer though

my buddy

left his full bottle of Liquid Trust in his car on a hot day, lid tightly closed yet the whole contents evaporated,

the stuff is as close to full alcohol as i can imagine, i have issues with NPA and evaporation, but this LT is

ridicules

jv, the marketing and packaging of LT is very well thought out, i agree, very slick...

[Archetypical Hybrid (HEC)]

I guess the

million-dollar question here is: would you consumers like to see oxytocin added to products such as our , or

by JVK with his SOE?

Vote please! Yay or Nay.. Include supporting comments if possible..

[WorkingMann]

I would say no..!
If I want

the LT, I'll buy on the side and add it myself.. Then I can get my right ratio also..
But I'm also not sure if it

works as I want and then if LT is added to other products these would be more expencive and I wouldn't wanna pay

more for that..
Also because some people may not want the LT mixed with their SOE, and if you want that you can

just buy the LT and mix yourself..
And if the results is LT should be mixed then I would say if we can't have the

"old" products and the "new"..
Hereby I mean:
"old" : SOE
"new" : SOE + LT
Because then we can those ourselfes

what we want..
Also because many people are having lots of succes with the products as they are now so we have to

keep them also even if it's decided that LT should be mixed with other products..

[xvs]

From Wikpedia: (because

even I had to check my assumptions)
"a peptide is an amino acid molecule without secondary structure; on gaining

defined structure, it is a protein." Thus the same molecule can be either a peptide or a protein depending on its

environment, though there are peptides that cannot be

proteins."

JVK


Don't trust Wikipedia too much. What we're

talking about here are polypeptides, not peptides. A peptide is a single amino acid residue (the amino acid as it is

once it's part of a chain), and a polypetide is two or more amino acids linked together.

Oxytocin is a

9-unit polypeptide.

All proteins are polypeptides, but the primary distinguishing factor between

polypeptides and proteins is whether they have tertiary structure, and this is determined mainly by the number of

peptides. The smallest human proteins which have been identified are about 45 peptides in length.

Proteins

have three structures:

- primary, which is the sequence of peptides (amino acids) in the chain.
-

secondary, which is the linkage between peptides in one part of the chain with peptides in the other part of the

chain (usually through the same hydrogen bonds that keep water liquid at room temperature).
- tertiary, which is

the complete structure of a protein, including all the folding (beta sheets, alpha helixes, etc.)

Oxytocin is

only 9 units long. This is too short to have ANY tertiary structure. So oxytocin is NOT a protein.

But it

does have a secondary structure, as can be seen in this paper:

secondary structure of part of

oxytocin (http://www.pubmedcentral.nih.gov/pagerender.fcgi?artid=286095&pageindex=4). The dotted lines are hydrogen bonds.

[jvkohl]

Don't trust Wikipedia

too much. What we're talking about here are polypeptides, not peptides. A peptide is a single amino acid residue

(the amino acid as it is once it's part of a chain), and a polypetide is two or more amino acids linked together.



Oxytocin is a 9-unit polypeptide.

xvs,
Someday soon we should speak on the phone. I know

that Wikipedia is not the ultimate source, but it's a reasonably good source for info that's easier to understand

than most--especially textbooks.

My primary interest is in the decapeptide hormone: gonadotropin releasing

hormone, which oddly enough has a 9-unit version of its mammalian counterpart in yeast, which is non other that the

alpha mating pheromone of "brewer's" yeast. I think that hormones are considered proteins, but could be wrong--and

also that their receptors are proteins, but could be wrong. We may just be running into definitions that don't help

researchers communicate well across different disciplines. Also, a fraction of the GnRH decapeptide appears to

function directly as a neurotransmitter: a hormone? a protein?

JVK

[maxo-texas]

Very little response from LT

if any.

Maybe one "trancy" session but get a similar feeling with light spanking (so endorphin flow

feeling).

Will be trying other new stuff and probably not reorder LT.

SOE seems to create enough trust.

[Archetypical Hybrid (HEC)]

JVK: I'm

curious.. What is the purity (assay) of the alpha-androstenol you use in SOE?? (>98%)??